Reporter

Part:BBa_K1718003:Design

Designed by: Hannah Young   Group: iGEM15_CU_Boulder   (2015-09-14)


N-acyl-homoserine-lactone inducible LuxPr promoter generating RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1642
    Illegal AgeI site found at 1754
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004


Design Notes

We removed the promoter from BBa_J04450. The TetR promoter and LuxR gene are necessary for induction of the promoter.


Source

The LuxR part is BBa_F2620 from the 2015 iGEM Distribution Kit. RFP or BBa_J04450 was also obtained from the 2015 iGEM Distribution Kit.

References